Essential Recombinant Human/cynomolgus Vegfa/vegf165 Protein (active) And More [M0DPNvez]

Essential Recombinant Human/cynomolgus Vegfa/vegf165 Protein (active) And More [M0DPNvez]

This high-quality recombinant protein, derived from human and cynomolgus cells, is a potent mediator of angiogenesis and vasculogenesis. The VEGFA/VEGF165 protein is a glycosylated mitogen that specifically targets endothelial cells, offering a wide range of applications in research and development. With its active form and specific sequence Met 1-Arg191, this protein is ideal for use in cell proliferation assays and various biological studies.

Product Properties and Specifications

The VEGFA/VEGF165 protein is produced in HEK293 cells, ensuring high purity and stability. It boasts a calculated molecular weight of 19.2 kDa and an observed molecular weight of 20-22 kDa. This protein is >95% pure, as determined by reducing SDS-PAGE, and has an endotoxin level of <1.0 EU per μg, as tested by the LAL method. The protein is lyophilized in a sterile buffer containing 100 mM Glycine, 10 mM NaCl, and pH 7.0, with added protectants like trehalose, mannitol, and Tween 80 for stability.

Applications and Benefits

Our Recombinant Human/Cynomolgus VEGFA/VEGF165 Protein is a valuable tool for researchers studying angiogenesis, vasculogenesis, and endothelial cell growth. Its bio-activity is measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC), with an ED50 typically ranging from 4-16 ng/mL. This protein is suitable for various applications, including cell culture, in vitro assays, and research into tumor growth and microvascular permeability. By using this high-quality protein, researchers can advance their studies and contribute to the understanding of vascular biology and related diseases.