Transform Recombinant Mouse M-csf Protein(n-his)(active) Mega Pack [8d2Uz6Xu]

Recombinant Mouse M-CSF Protein: A Potent Tool for Research

The Recombinant Mouse M-CSF protein (N-His)(active) is a highly purified and potent tool for research in the field of immunology and hematopoiesis. This recombinant protein is produced in E. coli and is tagged with a C-His tag, ensuring high purity and activity. With a calculated molecular weight of 19.0 kDa and an observed molecular weight of 17-25 kDa, this protein is ideal for various applications, including cell culture, ELISA, and Western blotting. Its bio-activity is measured by its ability to induce proliferation in NFS-60 cells, with an ED₅₀ of <2 ng/mL, making it a powerful tool for researchers.

Key Features and Specifications

This recombinant M-CSF protein is characterized by its high purity, exceeding 98% as determined by reducing SDS-PAGE. It is also endotoxin-free, with less than 0.1 EU per μg of the protein, ensuring minimal interference in your experiments. The protein is lyophilized from sterile PBS, pH 8.0, and contains 5% - 8% trehalose, mannitol, and 0.01% Tween 80 as protectants. This formulation ensures stability and ease of use. The protein is shipped with ice packs to maintain its integrity during transportation.

Applications and Benefits

The Recombinant Mouse M-CSF protein is a versatile tool for studying the role of macrophage colony-stimulating factor in various biological processes. It is particularly useful for researchers investigating the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. This protein can also be used to study the interaction between M-CSF and its membrane receptor, which modulates the proliferation of earlier hematopoietic progenitors and influences numerous physiological processes. With its high purity, bio-activity, and ease of use, this recombinant M-CSF protein is an essential addition to any research laboratory focused on immunology and hematopoiesis.