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Recombinant Swine TGF beta 1 Protein (N-His) (Active)

Our Recombinant Swine TGF beta 1 protein (N-His) (active) is a high-quality recombinant protein derived from E. coli expression system. This active protein is ideal for various research applications, including cell culture, immunology, and molecular biology studies. With its high purity and bio-activity, this product offers a reliable and efficient tool for your research needs.

Product Properties and Specifications

This recombinant protein is expressed with a C-His tag, which facilitates purification and detection. It has a calculated molecular weight of 13.7 kDa and an observed molecular weight of 11-17 kDa. The protein is >98% pure as determined by reducing SDS-PAGE and contains less than 0.1 EU per μg of protein as determined by the LAL method. This active protein has been shown to inhibit IL-4-induced proliferation in HT-2 cells with an ED₅₀ of <0.1 ng/mL, demonstrating its potent bio-activity. The protein is lyophilized from a sterile buffer containing sodium citrate, NaCl, and pH 4.5, with additional protectants like trehalose, mannitol, and Tween 80 added to ensure stability and reactivity.

Practical Applications and Benefits

The Recombinant Swine TGF beta 1 protein (N-His) (active) is a versatile tool for researchers studying the TGF-beta family and its role in cellular processes. This protein is particularly useful for investigating cell proliferation, differentiation, and apoptosis in various cell types. It can also be employed in the study of bone remodeling and osteoblastic bone formation. With its high purity, low endotoxin levels, and potent bio-activity, this product is a valuable asset for any laboratory. Whether you are a researcher in academia or industry, this recombinant protein can help you advance your research and contribute to the understanding of complex biological processes.