Top Pick Tev Protease, Recombinant, His-tag | Affinity Tag Removal Add To Cart [GR0lcEWp]

TEV Protease: The Ultimate Tool for Fusion Protein Tag Removal

Amid Biosciences' TEV Protease is a highly specialized and efficient tool designed for the removal of affinity tags from fusion proteins. This recombinant TEV protease, derived from the Tobacco Etch Virus (TEV), is engineered to offer unparalleled specificity and activity. With its optimal recognition site for the ENLYFQ(G/S) sequence, it ensures precise and targeted cleavage, making it an ideal choice for post-purification tag removal. The enzyme's high specificity minimizes off-target effects, ensuring a clean and pure protein sample.

Key Product Properties and Specifications

The TEV Protease is a 27 kDa protein, optimized for optimal performance at 30°C, though it can also be used at lower temperatures like 4°C. It contains an N-terminal His tag, which facilitates its removal from the digested protein samples using immobilized metal affinity chromatography (IMAC). The enzyme is highly stable, offering prolonged protease activity, and is compatible with both in-solution and on-column cleavage reactions. Purity is guaranteed, with the TEV Protease exceeding 90% purity as visualized on an SDS-PAGE gel.

Practical Applications and Target Audience

This versatile TEV Protease is an essential tool for researchers in various fields, including biotechnology, pharmaceuticals, and academia. Its high specificity and activity make it ideal for removing affinity tags from fusion proteins, simplifying downstream applications. The broad temperature and pH range further enhance its versatility. With a unit activity of 10,000 U/ml, it provides efficient and reliable results. Whether you are a seasoned researcher or a beginner in the field, the TEV Protease is your go-to solution for achieving high-quality protein samples with ease.