Customer Favorite N-his-ptrham Fast Cloning Kit | Toxic Protein Expression Take An Extra 75% Off [ZIMLRCdg]

Customer Favorite N-his-ptrham Fast Cloning Kit | Toxic Protein Expression Take An Extra 75% Off [ZIMLRCdg]

The N-His-pTrham Fast Cloning Kit from Amid Biosciences is a cutting-edge tool designed to streamline the process of rapid cloning and gene expression in E. coli. This comprehensive kit is tailored for researchers seeking efficient and precise methods to express toxic proteins. With its innovative features and user-friendly design, the kit offers unparalleled convenience and reliability.

Product Features and Specifications

The N-His-pTrham Fast Cloning Kit includes a linearized N-His-pTrham vector, which allows for the cloning of any PCR product. It also features E. coli AB 5-alpha Chemically Competent Cells for cloning and protein expression, control insert DNA, and sequencing primers for clone analysis. The kit components are as follows:

• Linearized N-His-pTrham (20 ng/µl): 10 µl
• Positive control: a PCR amplicon of GFP, 753 bp (20 ng/µl): 5 µl
• Sequencing Primers:
• Forward pTrham (5 µM): 10 µl
• Reverse pTrham (5 µM): 10 µl
• E. coli AB 5-alpha chemically competent cells: 5 X 100 µl

This kit is based on the pTrham vector, which offers the highest levels of expression for recombinant proteins. The vector includes the rhaP_BAD promoter, rrnG antitermination region, bacteriophage T7 gene 10 translation enhancer, and ribosome binding site. It also contains an ampicillin resistance marker, ensuring ease of selection and confirmation of clones.

Practical Uses and Benefits

The N-His-pTrham Fast Cloning Kit is an essential tool for researchers working with toxic proteins, providing a streamlined approach to cloning and expression. Its features make it highly versatile, allowing for the cloning of any PCR product. The kit is incredibly easy to use, requiring only a simple mix and transform process. It eliminates the need for enzymatic steps after PCR amplification, ensuring a faster and more efficient workflow. The system is seamless, with no extra nucleotides added, resulting in accurate and reliable results. With an efficiency of over 95% positive colonies, this kit is both economic and highly effective. By choosing the N-His-pTrham Fast Cloning Kit, researchers can save time and reagents while achieving high-quality results in their toxic protein expression studies.