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HRV 3C Protease: A Versatile Tool for Fusion Protein Cleavage

Amid Biosciences offers the HRV 3C Protease, a recombinant cysteine protease derived from human Rhinovirus type 14, known as PreScission Protease. This enzyme is designed for the precise cleavage of fusion proteins, making it an essential tool for researchers in various scientific fields. With its ability to specifically target the recognition sequence LeuGluValLeuPheGln/GlyPro, the HRV 3C Protease ensures efficient and reliable protein processing. The inclusion of a polyhistidine (6xHis) tag allows for easy removal of the enzyme from reaction solutions, enhancing purification and overall experimental workflow. This product is a game-changer for those seeking a high-performance protease with exceptional specificity and ease of use.

Product Specifications and Technical Details

The HRV 3C Protease is a 21 kDa protein, supplied in a convenient solution containing 20 mM Tris HCl (pH 7.5), 0.1 M NaCl, 2 mM 2-mercaptoethanol, 0.04% Tween 20, and 50% (V/V) glycerol. With an activity of ≥1000U/mg and a concentration of 1.1 mg/mL, this protease delivers exceptional performance. The unit definition of HRV 3C Protease is based on its ability to cleave ≥95% of the control fusion protein in 16 hours at 4°C. This product is designed for on-column or off-column cleavage, offering flexibility in experimental design. Its low-temperature activity ensures minimal disruption to the protein structure, making it ideal for sensitive applications.

Applications and Benefits of HRV 3C Protease

The HRV 3C Protease is widely applicable for the removal of purification tags from fusion proteins, providing a crucial step in protein purification workflows. Its low-temperature cleavage capability is particularly beneficial for maintaining protein stability and minimizing denaturation. The enzyme's active performance in a variety of commonly used buffers further enhances its versatility. The inclusion of on-column or off-column cleavage options allows for flexibility in experimental setup, while the ease of tag removal through immobilized metal affinity chromatography (IMAC) streamlines the purification process. Whether you are a researcher in academia or industry, the HRV 3C Protease is a valuable addition to your toolkit, offering reliable and efficient protein processing solutions.