Name: Super Saver Affings® Hyperactive Universal Cut&tag Assay Kit For Illumina Full Package [gXF1tWqG]
Brand: technovaprecision.com
SKU: HBS17971654311
Price: 62.99 USD
Availability: InStock
Rating: 4.04 (804 reviews)

Super Saver Affings® Hyperactive Universal Cut&tag Assay Kit For Illumina Full Package [gXF1tWqG]

ffiNGS® Hyperactive Universal CUT&Tag Assay Kit for Illumina

The AffiNGS® "Hyperactive Universal CUT&Tag Assay Kit for Illumina" is a reagent kit specifically developed for use with the Illumina high-throughput sequencing platform, designed for the targeted study of protein-DNA interactions. The CUT&Tag (Cleavage Under Target & Tagmentation) technique is a novel method for investigating protein-DNA interactions. It utilizes a transposase enzyme fused with Protein A/G, which precisely targets the desired protein under the guidance of antibodies and fragments DNA near the target site. This kit has been optimized for experimental reaction systems and library construction processes.

Compared to traditional ChIP-Seq (Chromatin Immunoprecipitation followed by Sequencing), this kit offers several advantages, including lower cell input requirements, shorter experimental timelines, higher signal-to-noise ratios, and excellent repeatability. It is particularly well-suited for research in areas such as early embryonic development, stem cells, cancer, and epigenetics. All reagents in the kit have undergone rigorous quality control and functional validation, ensuring maximum stability and repeatability in library construction.

Components:

	Components	AFG-VZ-TD903-01 12 rxns	AFG-VZ-TD903-02 48 rxns
Box 1	ConA Beads	130 µl	520 µl
	DNA Extract Beads	250 µl	1 ml
	Buffer L/B	2 X 750 µl	6 ml
	Buffer WA	1,5 ml	6 ml
	Buffer WB	1,5 ml	6 ml
Box 2	pA/G-Tnp (2 μM)	24 µl	96 µl
	5% Digitonin	150 µl	600 µl
	10 × Binding Buffer	400 µl	2 X 800 µl
	NE Buffer	1,2 ml	4 X 1,2 ml
	10 × Wash Buffer	2 X 1 ml	8 ml
	10 × Dig-300 Buffer	1,2 ml	4 X 1,2 ml
	Antibody Buffer (-)	1 ml	4 X 1 ml
	5 × TTBL	150 ml	600 µl
	Proteinase K	80 µl	320 µl
	2 × CAM	600 µl	2 X 1,2 ml

The colors indicated in the product component table represent the colors of the respective component tube caps:

pA/G-Tnp = Hyperactive pA/G-Transposon
NE Buffer = Nuclear Extract Buffer
TTBL = Trueprep Tagment Buffer L
CAM = CUT&Tag Amplification Mix

Storage:

Here are the storage conditions for the components of the kit:

BOX 1:

ConA Beads and DNA Extract Beads should be stored at 2 ~ 8°C.
The remaining components should be stored at room temperature (15 ~ 25°C).
Adjust the shipping method based on the destination temperature.

BOX 2:

5% Digitonin should be stored at -30 ~ -15°C and can be stored at room temperature (15 ~ 25°C) for up to 1 week.
The other components should be stored at -30 ~ -15°C.
During transportation, keep all components at or below 0°C.