Name: Non-toxic Recombinant Human Fgf Basic/fgf2/bfgf, Gmp Protein Cyber Monday Only [0KeZxYpH]
Brand: technovaprecision.com
SKU: HBS17828739396
Price: 31.99 USD
Availability: InStock
Rating: 3.93 (493 reviews)

Non-toxic Recombinant Human Fgf Basic/fgf2/bfgf, Gmp Protein Cyber Monday Only [0KeZxYpH]

Basic fibroblast growth factor (bFGF) is a member of the fibroblast growth factor (FGF) family. It is a growth factor and signaling protein encoded by the FGF2 gene. FGF family members have a wide range of mitogenic and cell survival activities and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. In normal tissues, bFGF is present in the basement membrane and the extracellular matrix under the endothelium of the blood vessels. bFGF can stimulate and regulate the differentiation and proliferation of various cells originating from the mesoderm and neuroectoderm, such as vascular endothelial cells, epithelial cells, myoblasts, osteoblasts and glial cells, and plays an important role in embryonic development and tissue healing. bFGF/FGF-2 is an essential component for maintaining pluripotency in all embryonic and induced pluripotent stem cell culture media.

This product is expressed by Escherichia coli and provided in liquid form with high activity, high purity, low endotoxin and no labeling.

Performance parameters

Synonyms	bFGF, FGF basic, FGF2, FGF-2, fibroblast growth factor 2 (basic), HBGF-2, Prostatropin.
Source	Human bFGF Protein expressed in E.coli.
Tag	No Tags.
Sequence	Ala 144 - Ser 288.
Molecular Weight	Approximately 16.29 kDa.
Accession	P09038.
Biological Activity	The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 1 ng/mL, corresponding to a specific activity of > 5.0 × 10⁶IU/mg.
Purity	> 95% as determined by SDS-PAGE and HPLC.
Endotoxin	＜0.01EU/μg protein as determined by LAL method.
Sterility	Negative.
Mycoplasma	Negative.
Vitro Virus	Negative.
Host Cell Protein	Less than 0.5 ng/ug when tested by ELISA.
Host Cell DNA	Less than 20 ng/mg when tested by qPCR.
Formulation	Liquuid from 0.22 μm filtered solution in PBS.
Physical Appearance	Liquid.

Storage conditions

Store at -85~-65℃, valid for 2 years.

Precautions

1. For your safety and health, please wear lab coat and disposable gloves when operating.

2. This product is for scientific research purposes only.

Figure

SDS-PAGE

Human FGF basic/FGF2/bFGF on SDS-PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human bFGF is greater than 95% as determined by SEC-HPLC.

Cell Based Assay

The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 1 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg.