Get Your Human Il-17r Alpha/cd217 Protein, His Tag Essentials [GCkiUEp5]

Source

Human IL-17R alpha/CD217 Protein is expressed from HEK293 with His tag at the C-terminal. It contains Leu33-Trp320.[Accession | Q96F46-1]

Molecular Weight

The protein has a predicted MW of 34.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per ug by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE and HPLC

Activity

ELISA Data: Immobilized Human IL-17R alpha, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-17A, His Tag with the EC50 of 0.15μg/ml determined by ELISA  

SPR Data:Human IL-17R alpha, His Tag immobilized on CM5 Chip can bind Human IL-17A, His Tag with an affinity constant of 1.57 nM as determined in SPR assay (Biacore T200).

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.