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On Sale Human Tpa Protein, His Tag Limited Stock [MaDqlk0X]
Product PropertieAlternative Namest-PA, tPA, Alteplase, Reteplase, PLAT,SpeciesHumanExpression SequenceSer36-Pro562, which consists of two chains: chain A (Ser36-Arg310) and chain B (Ile311-Pro562)AccessionP00750-1TagC-HisExpression SystemHEK293Molec
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On Sale Human Tpa Protein, His Tag Limited Stock [MaDqlk0X]
Product Propertie
|
Alternative Names |
t-PA, tPA, Alteplase, Reteplase, PLAT, |
|
Species |
Human |
|
Expression Sequence |
Ser36-Pro562, which consists of two chains: chain A (Ser36-Arg310) and chain B (Ile311-Pro562) |
|
Accession |
P00750-1 |
|
Tag |
C-His |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 60.13 kDa. The protein is activated by thermolysin and cleaved to a 2-chain protease, it migrates to 40-45 kDa (Chain A) and 35-40 kDa (Chain B) based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in 20mM MES, 300mM Nacl, 200mM Arginine (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Reconstitution Method |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM MES, 300mM Nacl, 200mM Arginine (pH 5.5). |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months.
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