Going Fast Human Tpa Protein, His Tag Save 75% Now [CIT2qbs0]

Alternative Names

t-PA, tPA, Alteplase, Reteplase, PLAT,

Species

Human

Expression Sequence

Ser36-Pro562, which consists of two chains: chain A (Ser36-Arg310) and chain B (Ile311-Pro562)

Accession

P00750-1

Tag

C-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 60.13 kDa. The protein is activated by thermolysin and cleaved to a 2-chain protease, it migrates to 40-45 kDa (Chain A) and 35-40 kDa (Chain B) based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in 20mM MES, 300mM Nacl, 200mM Arginine (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Reconstitution Method

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM MES, 300mM Nacl, 200mM Arginine (pH 5.5).