Official Ca-cy5 + Gift Included [8XlJRG9j]

HaloTag is a self-labeling protein tag engineered from the haloalkane dehalogenase DhaA, originally isolated from Rhodococcus rhodochrous. Its active site binds chloroalkane (CA)substrates, forming an irreversible covalent bond. This stable linkage between the HaloTag protein and its ligand enables rapid and efficient isolation and purification of tagged proteins. Official Ca-cy5 + Gift Included [8XlJRG9j] is a HaloTag ligand with Cyanine5 dye. Cy5 (Cyanine 5, 649Ex/710Em) dyes are known for their strong fluorescence, good photostability, and broad absorption spectra. The far-red emission minimizes interference from cellular autofluorescence. Cy5 can be used in combination with other fluorescent dyes (e.g., Cy3, GFP) to enable multicolor imaging, where multiple targets can be visualized simultaneously.

References

Wilhelm, J., Kuhn, S., Tarnawski, M., Gotthard, G., Tunnermann, J., Tanzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein, J., Hiblot, J., & Johnsson, K. (2021). Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry, 60(33), 2560–2575. https://doi.org/10.1021/acs.biochem.1c00268

Documents 

Datasheet          MSDS

Citations

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