Must-have Mouse Beta Nerve Growth Factor (ngf) Elisa Kit Set [7nR8W2fb]

This Must-have Mouse Beta Nerve Growth Factor (ngf) Elisa Kit Set [7nR8W2fb] from Innovative Research is intended for quantitative detection of mouse NGF in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.

This mouse NGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for NGF has been precoated onto 96-well plates. Standards(NSO, S122-G241) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse NGF amount of sample captured in plate.

• Detection Target: Beta Nerve Growth Factor (NGF)
• Uniprot ID: P01139)
• Reactivity: Mouse
• Cross-Reactivity: There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4.
• Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <1pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information:

The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.; Immunogen sequence: 300