Easy To Use Cd73 Monoclonal Antibody (ebioty/11.8 (ty/11.8)), Novafluor™ Red 725 Upgraded Model [oVidcCKs]

CD73 Monoclonal Antibody (eBioTY/11.8 (TY/11.8), NovaFluor™ Red 725)

The CD73 Monoclonal Antibody (eBioTY/11.8 (TY/11.8), NovaFluor™ Red 725) is a powerful tool for researchers studying CD73, a 69-kDa GPI-anchored cell-surface protein with ecto-5'-nucleotidase activity. This antibody is specifically designed to recognize CD73 on myeloid cells, particularly in the bone marrow, and has been shown to be a valuable asset in understanding the role of CD73 in mediating activation signals. Key features of this antibody include:

• High specificity for CD73 on myeloid cells
• Expression restricted to bone marrow in myeloid cells
• Induces secretion of a soluble form with IL-2, suggesting its role in activation signals

Product Features and Specifications

The eBioTY/11.8 (TY/11.8) antibody is ideal for flow cytometric analysis, providing reliable and consistent results. It has been tested and validated for use with mouse splenocytes, making it suitable for a wide range of research applications. Key features and specifications include:

• Excitation at 636 nm; emission at 727 nm
• Compatibility with NovaFluor™ Red 725 dye for enhanced fluorescence
• Recommended for use with CellBlox Blocking Buffer for optimal staining results

This antibody is a valuable addition to any laboratory's toolkit for studying CD73 and its role in cellular signaling and activation.

Applications and Practical Uses

The CD73 Monoclonal Antibody (eBioTY/11.8 (TY/11.8), NovaFluor™ Red 725) is highly versatile and can be used in various research applications, including the study of CD73 expression on lymphocytes, particularly during lymphocyte maturation. It has been found that memory CD4 T cells express CD73 and are similar to uncommitted primed precursor helper cells, which can differentiate into Th1 or Th2 cells. Applications include:

• Flow cytometric analysis of CD73 expression on myeloid and lymphoid cells
• Studying the role of CD73 in activation signals and cellular differentiation
• Understanding the differences between human and mouse CD73 expression patterns

With its exceptional performance and wide range of applications, this antibody is an essential tool for researchers in immunology, cell biology, and related fields.