Elegant Soilwater: Bar Medium ~ Limited Availability [DUNOqob8]
Elegant Soilwater: Bar Medium ~ Limited Availability [DUNOqob8]Suitable for some mixotrophic and most heterotrophic (colorless) euglenoids.DirectionsFor 200 mL TotalCombine components.Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradua
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Elegant Soilwater: Bar Medium ~ Limited Availability [DUNOqob8]
Elegant Soilwater: Bar Medium ~ Limited Availability [DUNOqob8]
Suitable for some mixotrophic and most heterotrophic (colorless) euglenoids.
Directions
For 200 mL Total
- Combine components.
- Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 °C in 15 minutes. Then increased just over 98 °C for the 3 hour duration. Cooling occurs gradually at room temperature.
- Refrigerate 24 hours or more and bring to room temperature before use.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | Green House Soil | 1 tsp | ||
| 2 | Barley grains (add after dH2O) |
3 grains | ||
| 3 | CaCO3 (optional) (Fisher C 64) |
1 mg | 0.05 mM | |
| 4 | dH2O | 200 mL |
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