Most Popular Recombinant Human Alkaline Phosphatase/alpl Protein (his Tag)(active) Guaranteed [SesCE2Ux]

Most Popular Recombinant Human Alkaline Phosphatase/alpl Protein (his Tag)(active) Guaranteed [SesCE2Ux]

Experience the precision and reliability of our Most Popular Recombinant Human Alkaline Phosphatase/alpl Protein (his Tag)(active) Guaranteed [SesCE2Ux]. This high-quality, active protein is a crucial tool for various research applications. Synthesized through recombinant DNA technology in HEK293 cells, this protein offers exceptional purity and functionality. Let's delve into its key features and benefits.

Key Features:

• Purity: Our ALPL protein is >95% pure, as determined by reducing SDS-PAGE, ensuring reliable and consistent results.
• Host Cell: Derived from HEK293 cells, this protein maintains high biological activity and is suitable for a wide range of applications.

Applications:

• Research Applications: Ideal for enzyme kinetics studies, protein purification, and as a control protein in various biochemical assays.
• Pharmaceutical Development: Utilize this protein to assess the efficacy of inhibitors or activators in the context of alkaline phosphatase activity.

Product Specifications:

This recombinant protein is expressed as a His-tagged fusion protein, providing ease of purification and identification. The sequence covers Met 1-Ser 502, with a calculated molecular weight of 55.0 kDa and an observed molecular weight of 65 kDa. Our ALPL protein is active, as evidenced by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP), with a specific activity > 50,000 pmoles/min/μg.

Properties and Storage:

• Purity: >95% as determined by reducing SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Storage: Lyophilized protein is stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days.

Reconstitution: Please refer to the printed manual for detailed instructions.